This study compared heart rate variability (HRV) and stress index changes across four major activities: humming (Bhramari Pranayama), physical activity, emotional stress, and sleep. The results showed that humming lowered the stress index more than any other activity, and its HRV improvement effects were clearly demonstrated. These findings show that a simple daily humming habit can effectively promote autonomic nervous system health and stress relief.
1. Research Objectives and Background
The purpose of this study was to determine whether humming (simple Bhramari Pranayama) actually reduces stress and how it affects autonomic nervous system balance, and to compare HRV changes during physical activity, emotional stress, and sleep over an extended period.
"Humming was found to produce the lowest stress index compared to physical activity, emotional stress, and sleep."
The Negative Effects of Stress and HRV
Stress is closely linked to the worsening of cardiovascular disease, diabetes, pulmonary disease, and cancer, raising not only quality of life concerns but actual health risks. Furthermore, HRV (heart rate variability) is an important indicator of autonomic nervous system health -- higher HRV means better stress coping and better health.
"Relaxation, slow breathing, or any stress-reducing intervention increases HRV."
2. Effects of Humming (Bhramari Pranayama) and the Need for Research
Humming is a simple yet multifaceted practice with various benefits:
- Reduced heart rate
- Increased HRV
- Improved autonomic and pulmonary function
- Improved attention and sleep quality
...all of which have been demonstrated in multiple studies.
This study aimed to confirm how distinctive the effects of Bhramari humming are compared to various activities throughout the day, and whether the effects appear consistently across multiple individuals.
"It was observed that HRV indices improved noticeably through humming exercises related to HRV biofeedback. These changes do not appear during stress or physical activity."
3. Research Methods and Participants
Study Design and Participants
- Method: HRV comparison across 4 activities (humming, physical activity, emotional stress, sleep) measured via Holter monitor for 16+ hours
- Participants: 23 of 29 men and women aged 18-60 provided final data (average age 38.8, some with chronic conditions)
Key Activity Definitions
- Humming (Bhramari): Inhale for 3-4 seconds, slowly exhale while making a humming sound for 6-8 seconds (15+ minutes)
- Physical activity: Walking, running, cycling, etc.
- Emotional stress: Moments such as traffic congestion, emotionally difficult phone calls, etc. (self-reported)
- Sleep: Nighttime sleep (excluding naps)
Each participant completed an activity log, and HRV measurement results were verified by experts.

4. Key Analysis Results
HRV & Stress Index Comparison
Key findings summary:
- Stress Index (SI) during humming was the lowest among all activities (significantly lower than even sleep, p<0.00073)
- SDNN (overall autonomic variability) and Total Power were also highest during humming
- Heart rate during humming: Significantly lower than during physical activity and stress, second lowest after sleep
- RMSSD (parasympathetic variability) was higher during humming than during physical activity and stress situations, but not different from sleep
- LF/HF ratio: Highest during humming (a result of total power being concentrated in the LF band)
"Humming activity showed consistently unique improvements in stress index, SDNN, total power, and LF/HF ratio compared to all three other activities."
| Activity | Stress Index (SI) | Heart Rate | SDNN | Total Power | LF/HF |
|---|---|---|---|---|---|
| Humming | 9.26 (+/-2.49) * | 82.5 | 61.8 | 4318.8 | 13.5 |
| Physical Activity | 23.4 (+/-13.1) | 103.3 | 26.7 | 1003.8 | 5.24 |
| Sleep | 10.9 (+/-3.47) | 70.3 | 47.7 | 2750.9 | 2.26 |
| Stress | 21.7 (+/-7.39) | 97.2 | 22.4 | 648.4 | 5.70 |
"Both humming and sleep are effective at reducing stress, but humming produces an even lower stress index than sleep."
5. Discussion and Implications
Humming's Unique HRV Pattern
- Humming showed superior results across various HRV indices compared to physical activity and stress situations, and demonstrated considerable efficacy even compared to sleep.
- Despite the ambiguity in LF/HF ratio interpretation, total power during humming was concentrated in the LF band, showing a distinctive characteristic.
- Additionally, the increase in SDNN indicates potential benefits for concentration and autonomic nervous system balance.
"Most HRV indices (particularly excluding RMSSD) were higher during humming than even during sleep. This demonstrates humming's unique, intentional autonomic activation pattern."

Real-World Applicability and Future Research Directions
- Humming may be a way to more easily realize the positive effects of HRV biofeedback.
- Practicing humming for just 10-15 minutes twice daily is expected to yield various health benefits (autonomic nervous system, cardiovascular, respiratory, attention and focus, etc.).
- Future research is needed to compare HRV across different sleep stages and to clarify the effects of long-term habituation.
"By practicing humming for just 10-15 minutes twice a day, you can gain multiple benefits including improved HRV, strengthened autonomic nervous system, and reduced stress."
6. Limitations and Caveats
- Sample size: The number of participants was not large, so larger-scale studies are needed in the future.
- Differences in measurement duration across activities: Long-duration activities (sleep) and short-duration activities (humming/stress/physical activity) were not measured under strictly identical conditions, which limits comparisons.
- Ambiguity in LF/HF interpretation: Interpretation of the LF/HF ratio can vary depending on breathing and measurement environment, requiring caution in result interpretation.
Conclusion
This study experimentally demonstrated that simple humming (Bhramari Pranayama) can effectively reduce stress and improve autonomic nervous system health indicators such as HRV. Humming, which can be easily practiced in daily life, showed superior effects compared to sleep, physical activity, and even stress situations, suggesting it can be recommended as a healthy lifestyle strategy. We can look forward to how consistent practice and further follow-up research will contribute to health improvement.
"Humming is not only an effective stress relief method but can also contribute to improved HRV and enhanced quality of life."